Accumulation of Brilliant Cresyl Blue in the Sap of Living Cells of Nitella in the Presence of Nh3
نویسنده
چکیده
When the living cells of Nitella are placed in a solution of brilliant cresyl blue containing NH(4)Cl, the rate of accumulation of the dye in the sap is found to be lower than when the cells are placed in a solution of dye containing no NH(4)Cl and this may occur without any increase in the pH value of the cell sap. This decrease is found to be primarily due to the presence of NH(3) in the sap and seems not to exist where NH(3) is present only in the external solution at the concentration used.
منابع مشابه
Mechanism of the Accumulation of Dye in Nitella on the Basis of the Entrance of the Dye as Undissociated Molecules
The rate of penetration of brilliant cresyl blue into the living cells of Nitella indicates that the dye enters only in the form of the undissociated molecule. At equilibrium the total concentration of the dye in the sap is proportional to the concentration of the free base in the outside solution.
متن کاملOn the Accumulation of Dye in Nitella
Living cells of Nitella were placed in different concentrations of brilliant cresyl blue solutions at pH 6.9. It was found that the greater the concentration of the external dye solution, the greater was the speed of accumulation of the dye in the cell sap and higher was the concentration of dye found in the sap at equilibrium. Analysis of the time curves showed that the process may be regarded...
متن کاملStudies of the Inner and Outer Protoplasmic Surfaces of Large Plant Cells
The vacuolar surface of Nitella is covered with a non-aqueous film too thin to be visible as a separate membrane. The motion of the protoplasm may subject this film to a good deal of mechanical disturbance. Apparently this does not rupture the film for no dye escapes into the protoplasm as the result of such disturbance when the vacuolar sap is deeply stained with neutral red or brilliant cresy...
متن کاملA GLASS ELECTRODE APPARATUS FOR MEASURING THE pH VALUES OF VERY SMALL VOLUMES OF SOLUTION
A glass electrode apparatus is described with which pH measurements can be made with as small volumes as 2 drops (about 0.14 cc.) of solution. Using this apparatus the change of pH of the vacuolar sap of Nitella, due to the penetration of brilliant cresyl blue, has been readily followed. The sap and the dye have been found to poison the usual type of hydrogen electrode.
متن کاملThe Effect of Acetate Buffer Mixtures, Acetic Acid, and Sodium Acetate, on the Protoplasm, as Influencing the Rate of Penetration of Cresyl Blue into the Vacuole of Nitella
When living cells of Nitella are exposed to a solution of sodium acetate and are then placed in a solution of brilliant cresyl blue made up with a borate buffer mixture at pH 7.85, a decrease in the rate of penetration of dye is found, without any change in the pH value of the sap. It is assumed that this inhibiting effect is caused by the action of sodium on the protoplasm. This effect is not ...
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ورودعنوان ژورنال:
- The Journal of General Physiology
دوره 9 شماره
صفحات -
تاریخ انتشار 2003